Hepatoprotective activity of extracts of Sapindus mukorossi and Rheum emodi in CCl4 induced liver damage: In vitro and in vivo studies

Mohammed Ibrahim, Mohammed Nane Khaja, Aleem Ahmed Khan, Mohammed Aejaz Habeeb, Chitoor Mohammed Habibullah, Anjum Aara, Yalavarthy Prameela Devi, Mangamoori Lakshmi Narasu Lakshmi Narasu
1Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences and Allied Hospitals, Kanchanbagh, Hyderabad, Andhra Pradesh, India, 2Nizam Institute of Pharmacy, Deshmukhi, Pochampally, Hyderabad, Andhra Pradesh, India, 3Centre for Biotechnology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, Andhra Pradesh, India

Our aim was to study the hepatoprotective activity of S.mukorossi and R.emodi extracts in CCl4 treated male rats. The dried powder of S. mukorossi and R. emodi was extracted successively with petroleum ether, benzene, chloroform and ethanol and concentrated in vacuum. Rat hepatocytes in primary monolayer culture methods were used for in vitro study. In in vivo studies the hepatoprotective activity of the extract of the fruit pericarp of S. mukorossi and rhizomes of R. emodi was studied on liver damaged (CCl4 induced) male rats. In vitro study: CCl4 damaged primary monolayer culture of the hepatocytes was treated with the extracts of S. mukorossi and R. emodi. Hepatoprotective activity was observed in the CCL4 damaged primary monolayer culture. In vivo study: Extracts of the fruit pericarp of S. mukorossi (2.5mg/ml) and rhizomes of R. emodi (3.0mg/mld) were found to have reparative properties in CCL4 induced liver damaged rats as judged from serum marker enzyme activities. We can conclude from this study, that the extracts of S. mukorossi and R. emodi are hepatoprotective both in vitro and in vivo.