The Anti spasmodic effect of supermint extract in rat ileum in an in vitro model

Mitra Emami Abarghouei1, Maziar-mohamad Akhavan2, Abasali Vafaee3, Abasali Taherian4
1University of Medical Sciences, Semnan, Iran, 2University of Medical Sciences, Semnan, Iran, 3University of Medical Sciences, Semnan, Iran , 4University of Medical Sciences, Semnan, Iran

Previous studies indicated that supermint is a plant which is gaining decreasing acceptance as a folk remedy for treatment of the irritable bowel disease although the mechanism of this action is not clear yet. For this purpose we studied the effect of supermint extract in rat ileum Male Wistar rats (220-250 g) were used .pieces of ileum (20-30 mm) were mounted in a 50 ml organ bath containing Tyrode solution with temperature (37° C).and bubbled with (95%O2,5%CO2). The ileum was stimulated at 0.1 Hz and contraction was recorded by physiograph. Then supermint extract was added in five concentration and change in contraction calculated. It has been shown that 0.1 Hz stimulation of rat ileum induced contractions which are depressed by atropine. Addition of aqueous extract of supermint extract to the organ bath during 0.1Hz stimulation decreased contraction in a dose dependent manner (EC50=0.7mg/ml). it seems that supermint extract shows its effect by inhibition of the cholinergic system because the contraction known to be due to release of acetylcholine at 0.1Hz was decreased by addition of supermint extract.