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Adaptation and repair of the urothelium: Responding to both acute and chronic intravesical acrolein administration in the rat Acrolein (ACR), a metabolite of cyclophosphamide and ifosfamide, has been reported previously in patients to be urotoxic, causing erosion of the urothelial layer resulting in haemorrhagic cystitis (1). It has been previously demonstrated that acute and repeated administration of ACR directly into the bladder of rats induces an inflammatory cascade resulting in sensitisation of the urinary system, with bladder hyperactivity and an increase in voiding frequency observed (2). The present study was designed to determine the effects on micturition parameters in conscious rats (voiding frequency, total voided volume and average volume per void), urine cytokine levels, histopathology and the time course of recovery following intravesical administration of ACR.
Data are expressed as arithmetic mean ± s.e.m. (*p<0.05, **p<0.01, ***p<0.001 vs. VEH). Statistical testing of total volume data was a repeated analysis of covariance. Frequency data analysis was conducted using a Poisson model at each time point. When the bladders from these studies were examined histologically after acute and repeated ACR treatment repair was already underway with evidence of urothelial regrowth and fibrobast activation. Conclusion: Urological and histological evidence suggests bladder repair following ACR-induced cystitis.
1. Macedo F.Y. et al ., (2008) Exp Toxicol Pathol. 59 (6)425-30. |
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