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The 3H-CGP 12177 whole cell binding characteristics of the three turkey β-adrenoceptors There are three turkey β-adrenoceptors (AR) – turkey β (Aurbach etal., 1975), 3C and 4C (Chen et al., 1994). Although the turkey β-AR has similar sequence to the human β1-AR (Warne et al.,2008) it is not known whether the pharmacological binding characteristics are similar. The turkey β4C receptor has been reported to be unlike the turkey β-AR (Chen et al.,1994). No data have been published for the turkey β3C receptor. This study examined the binding characteristics of the 3 turkey β-ARs and compared them to the 3 human β-ARs. CHO-K1 cells were transfected with either the original full length turkey β-AR, the turkey β3C-AR or β4C-AR. The full length turkey β-AR did not express so a C-terminally truncated version was transfected (tβtrunc). Stable clonal cell lines were selected by dilution cloning. 3H-CGP 12177 whole cell binding was performed as previously described (Baker 2005). When the log KD values for a wide range of ligands are plotted against the log KD values from the human β-ARs (data from Baker 2005 and abstract at this meeting) it can be seen that the tβ3C receptor most closely resembles the human β2-AR Figure A). There are some marked differences between the tβtrunc receptor and the human β1-AR (Figure B) and the tβ4C receptor is unlike any of the human B-AR or other turkey β-ARs.
Table. Log KD values from 3H-CGP12177 whole cell binding.
JGB is a Wellcome Trust Clinician Scientist Fellow. |
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