011P University of Cambridge
The Twelfth International Conference on Endothelin 2011



Modulation of release of Endothelin-1 in hypertrophic human endocardial and vascular endothelial cells

Johny Al-Khoury1, Dima Abdel Samad1, Chantale Provost1, Sheldon Magder2, Pedro D’Orléans-Juste3, Ghassan Bkaily1, Danielle Jacques1. 1Faculty of medicine, University of Sherbrooke, Sherbrooke, Quebec, Anatomy and cell biology, J1H 5N4, Canada, 2McGill University Health Centre, McGill University, Montreal, Quebec, Critical Care Division, Royal Victoria Hospital, H3A 1A1, Canada, 3Faculty of medicine, University of Sherbrooke, Sherbrooke, Quebec, Pharmacology, J1H 5N4, Canada.


As vascular endothelial cells (VECs) line the vascular walls, endocardial endothelial cells (EECs) line the cardiac cavities and constitute a barrier between the circulating blood and the adjacent cardiomyocytes. In addition, similar to VECs, EECs produce and release various cardioactive factors such as Neuropeptide Y (NPY) and Angiotensin II (Ang II). Theses peptides are implicated in various cardiac pathologies including hypertrophy of cardiomyocytes. However, it is not known whether EECs and VECs volume is also increased and whether this modulates excitation-secretion coupling of these cells. Using real 3D confocal microscopy imaging, immunofluorescence and various molecular biology techniques, we found that NPY and Ang II induce an increase in the volume of human EECs and VECs. This increase in cell volume is accompanied by an increase in hypertrophic markers such as the DNA/protein ratio and ANP mRNA. We also found that Ang II-induced hypertrophy of EECs and VECs is primarily mediated via AT1 receptor activation whereas the NPY-induced hypertrophy is mediated via activation of the Y1, Y2 and Y5 receptors. In addition, NPY and Ang II modulate the release of Endothelin-1 from normal and hypertrophic human EECs and VECs. Thus, similar to cardiomyocytes, cardiac hypertrophy is associated with EECs and VECs hypertrophy and modulates the secretory capacity of these cells. Modulation of secretion may in turn contribute to the cardiac remodelling that occurs in hypertrophy and heart failure. The work was supported by the Canadian Institutes of Health Research (CIHR) and the Heart and Stroke Foundation of Quebec (HSFQ). The authors thank Quebec Transplant and the donors for the donated tissues.