Plasma adypokines in a Venezuelan population with newly diagnosed metabolic syndrome.

Y Mathison1,4, L Sánchez2,3, Y López2,3, L Figueira4, MR Garrido4, A Israel4. 1Universidad Central de Venezuela, Department of Pharmacology, School of Medicine José María Vargas, Venezuela, 2Hospital Vargas, Internal Medicine Service 3, Venezuela, 3Universidad Central de Venezuela, Department of Clinical Medicine A, School of Medicine José María Vargas, Venezuela, 4Universidad Central de Venezuela, Laboratory of Neuropeptides, School of Pharmacy, Venezuela

Obesity is associated with increased accumulation of T cells and macrophages in adipose tissue, which may play important roles in obesity-related disease. Activated macrophages and T cells within adipose tissue produce increased levels of inflammatory chemokines such as CCL2/ MCP-1 and CCL5/RANTES as well as cytokines such as tumor necrosis factor-α (TNF-α). Release of these mediators of inflammation is increased by the adipose tissue in obesity and may affect other organs involved in the metabolic syndrome by paracrine or endocrine effects. Moreover the dysfunction of adipose tissue can lead to imbalance between nitric oxide (NO) and oxidative stress. Therefore, we examined the relationships between adipokines, malonildialdehyde (MDA), nitric oxide and gluthation peroxidase activity and some features of metabolic syndrome, in a Venezuelan population with newly diagnosed metabolic syndrome. Bio-Plex Pro™, a magnetic bead-based multiplex assays designed to measure multiple cytokines, chemokines and growth factor was used to quantify simultaneously these parameters in human plasma samples. Plasma levels of IL-8, PDGF-bb, RANTES, TNF-α, MDA and GPx activity in patient with metabolic syndrome were significantly increased compared to those in control patients (p<0.05). MDA levels were positively correlated with RANTES (r=0.511, p<0.04) and hypertension (r= 0.497, p<0.008), and were negatively correlated with IL-8 (r= -416. p< 0.05). Our findings indicate that RANTES and IL-8 may be involved in the development of metabolic syndrome–related risk factors, and indicate an association of the disease with specific members of the chemokine family, oxidative stress and lipid peroxidation (Misión Ciencia, Proyecto ECCV No. 2007001585; CDCH PI-09-7414-2008)