P444

Peripheral expression of inflammatory markers in a venezuelan population with overweight

MR Garrido1, L Sanchez2, Y Lopez2, Y Mathison2, A Israel1. 1Universidad Central de Venezuela, Faculty of Pharmacy, Venezuela, 2Universidad Central de Venezuela, Faculty of Medicine, Venezuela

Cytokines are crucial immune mediators in many physiological and pathophysiological conditions. Chemokines have been hypothesized to be involved in macrophage infiltration into adipose tissue in obesity and might therefore play an important role in the development of obesity-related disorders like metabolic syndrome. The objective of this study was to evaluate the peripheral expression of inflammatory markers in a selected Venezuelan population and its correlation with parameters of metabolic syndrome. A group of 36 individuals, male and females, aged between 19–68 years, were divided into 3 groups: Normal weigh (NW), grade 1 obesity (OB1) and grade 2 obesity (OB2). The data related to the parameters of the metabolic syndrome such as glycemia, body mass index (BMI), microalbuminuria and the peripheral expression of circulating interleukins (IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, IL-8, IL-10); other cytokines such as tumor necrosis factor-alpha (TNFα), interferon-γ–inducible protein-10 (IP-10/CXCL10), interferon gamma and monocyte chemotactic protein-1 (MCP-1), and the systemic inflammation marker C-reactive protein (CRP) were evaluated. Bio-Plex Pro™, a magnetic bead-based multiplex assays designed to measure multiple cytokines, chemokines, and growth factors was used to quantify simultaneously this parameters in plasma samples. Higher values of TNF-alpha, IL-6, C-reactive protein, glycemia, BMI and microalbuminuria were found (NW