Antispasmodic activity of aqueous extract from the Mediterranean red seaweed, Hypnea musciformis on the rat isolated duodenum

M Rasgallah - Krouf, A Yacoub, W Chaouech, A Bouraoui. Faculté de Pharmacie, Université de Monastir, Pharmacologie et Sciences pharmaceutiques, 5000, Tunisia

The marine ecosystem with its rich diversity and abundance of natural products can contribute to the discovery of new potential drugs. The aim of this study was to investigate the effect of aqueous extract from the Mediterranean red seaweed, Hypnea Musciformis, on the rat isolated duodenum contraction. The aqueous extract was obtained by maceration of marine seaweed in distilled water followed by lyophilization of the filtrates. The final concentrations of the lyophilized extract, ranged from 0.5 to 3 mg/ ml, were made up daily in the physiological solution. All solutions of spasmogenic substances (acetylcholine and barium chloride) and of the reference antispasmodic drug (alverine) were made up daily from stock solutions. Wistar rats were Killed by cervical dislocation and duodenum of 1.5 cm length were removed , cleaned and placed in 20 ml organ bath containing Tyrode solution and maintained at 37°C and constantly oxygenated with 95% O2 + 5% CO2. Intestinal responses were recorded using myograph transducer and physiograph (MKIII, Narco Biosystems, USA). The antispasmodic activity of different concentrations of the aqueous extract was tested against two spasmogenic substances acetylcholine (Ach) and barium chloride (BaCl2). The test substances of aqueous extract from Hypnea Musciformis, were added to the bath in volume of 1 ml two minutes before the spasmogenic substances. In the first part of the experiments, cumulative dose-response curves of Acetylcholine and BaCl2 were obtained. Dose response curve were repeated after addition of different concentrations of the aqueous extract or of the reference drug, alverine We established that the aqueous extracts exhibited a significant inhibition of the contractile response induced by acetylcholine or by BaCl2. Indeed, contractions induced by acetylcholine were reduced by 36% (p<0.05), 56%(p<0.01) and 60% (p<0.001) when the aqueous extract was used at 1 mg/ml, 2 mg/ml and 3 mg/ml respectively. And, contractions induced by BaCl2 were reduced by 45% (p<0.05), 67%(p<0.01) and 90%(p<0.001) respectively in presence of the aqueous extract at concentration 0.5 mg/ml, 0.75 mg/ml and 1 mg/ml. In addition to its several biological and pharmacological activities (anti-inflammatory, anti-proliferative, antibacterial and antifungal), the present study revealed that this red seaweed, exhibited a significant antispasmodic activity in the tested in vitro model. It is well Known that the aqueous extract of red seaweed contains polar constituents (polyphenols and sulfated polysaccharides), so further studies must be conducted in order to clarify which constituent of this extract is responsible for this antispasmodic activity.

References: Ben Aoun et al, 2010; El Gamal et al, 2010 ; Bouhlal et al, 2010 ; Borgi et al, 2009; Saeidnia S et al, 2009 ; Siddiqui et al, 2009 ; Hayase et al, 1993.