The Role of Diallyl Trisul fi de as an Metastasis Suppressor in actived-platelets induced Human Breast Cancer MDA-MB-231 Cells adhesion, migration and invasion

Yuping Liu. Nanjing University of Chinese Medicine, Nanjing University of Chinese Medicine, 282 Hanzhong Road, Nanjing, 210029, China

Diallyl Trisulfide(DATS) is a widely used Chinese herbal medicine that has been used historically in anti-inflammatory ,anti-septicize and anti-cancer therapy. However, the molecular mechanism of its anti-cancer activity were focus on inhibit cell proliferation and promote apoptosis ,the effect of DATS on suppress tumor metastases remains poorly understood and warrants further investigations. Meanwhile platelets activation is an important agent during the tumor cells transfer from primarily site to another ,because the formation of platelets-tumor compounds can help tumor cell succeed in escaping from the attack of immunologic system. The purpose of this study is to verify the activity of DATS to inhibit active-platelets induced MDA-MB-231 human breast cancer cells metastasis. The results indicated that DATS inhibited platelets aggregation, decreased the release of TXB2 and increased 6-keto-PGF1α of platelets, it also suppressed MDA-MB-231 cell adhesion to active-platelets, wound healing mobility, migration and invasion through the rat tail collagen in a concentration-dependent manner. PCR, Western blot and enzyme linked immunosorbent assay showed that DATS significantly inhibited the expression and secretion of matrix metalloproteinases 2/9 (MMP-2/9) in MDA-MB-231 cells and promoted the expression of tissue inhibitor of metalloproteinase 1(TIMP1). Additionally, treatment of MDA-MB-231 cells with DATS down-regulated the expression of Integrin β1,3. Taken together, DATS had potential to suppress the adhesion, migration and invasion of MDA-MB-231 cancer cells in vitro and it could serve as a promising drug for the treatment of cancer metastasis.