Compound action potential parameters and ketoprofen

Jelena Scekic, Mara Drecun. Medical Faculty, Physiology, Montenegro

Introduction: Ketoprofen is non-steroidal antiinflammatory drug. The study aim is to examine effect of ketoprofen cream in solution form, on amplitude and latency periods, parameters of nerve compound action potential (CAP).

Materials: CAP parameters were measured using extracellular recording techniques, stimulator and digital storage oscilloscope. Frog sciatic nerves were devided into 3 groups, 8 nerves in each group. Nerves were incubated in Ringer solution for 2 hours and control measurements were done. Than, nerves were incubated for 20 min. in following solutions: control group: Ringer solution, group I: 0.075% and group II: 0.25% ketoprofen solution. CAP parameters were measured again. Data were analysed using the statistical computer programe GrapPadPrism 5.0.

Results: After 20 minute incubation in 0.25% and 0.075% ketoprofen solution amplitude of CAP decreased for 96.67 ± 9.43% and 29.51 ± 1.09%, onset latency period of the CAP increased for 90.33 ± 9.66% and 16.64 ± 5.32% and peak latency period of the CAP for 91.74 ± 8.25% and 22.08 ± 6.25%, in relation to control measurement value, respectively. Data were statistically significant in relation to control group for p<0.01.

All nerves recovered their function after 2 hours incubation period in Ringer solution.

Conclusion: Study results showed that ketoprofen solution statistically significant decreases the CAP amplitude and increases latency perods. Ketoprofen may have an antinociceptive effect since the latency periods represent the nerve impulse conduction time along fast and slower conducting fibers, conductivity along the corresponding fibers of peripheral nerve which could transfer pain signals. More precise investigations with pure substances are needed.