Characterization and functional significance of cannabinoid receptors in skeletal muscle

FM Shermado, Andrew Bennett, Kostas Tsintzas. The university of Nottingham, Nottingham, UK

Effect of ACEA and RIM on insulin stimulated AKT phosphorylation in Wister rat myotubes. A) Pre-treatment (24 hours) of myotubes with 100nM ACEA did not alter the acute (10min) stimulatory effect of 100 nM INS (insulin) on AKT phosphorylation. ** denotes P<0.01 compared to vehicle (0.01%). B) Pre-treatment (24 hours) of myotubes with 100nM RIM did not alter the acute (10min) stimulatory effect of 100 nM INS (insulin) on AKT phosphorylation. * ** denotes compared to vehicle (P<0.001). Protein expression (above) and Densitometric (bellow) of PAKT and TAKT. Data were analysed by one way ANOVA with Bonferroni post-hoc test.