Screening for radioprotective components of Glycosides and Alcohols from Rhodiola in vitro

Tian-Xiang Ma, Jiu-Hong Wu, Ning Shi, Hong-Zhu Gong. Department of Pharmacy,306 Hospital of PLA, Beijing, China

Abstract: Aim To comparative study the radioprotective activity of six glycosides or alcohols from Rhodiola in vitro, including Salidroside, Isoquercitrin, Tyrosol, Rosarin, Arbutin and Rosavin.

Methods: Lymphocytes (AHH-1) were exposed to 10 Gy 60Co γ-ray to induce radiation injury model, and then MTS assay was used to detect the proliferation activity of AHH-1 after treated by six kinds of glycosides or alcohols from Rhodiola. Results Compared with the normal group, 4 Gy, 6 Gy, 8 Gy and 10 Gy model groups showed obvious low survivability of AHH-1. And the survival rate of 10 Gy model group was (55.43 ± 4.69) %. Compared with the model group, Isoquercitrin (12.5 μg.mL-1, P < 0.05), Salidroside (12.5 μg.mL-1, P < 0.05) , Rosavin (12.5 μg.mL-1, P < 0.01), Rosarin (25 μg.mL-1, P < 0.05), Arbutin (12.5 μg.mL-1, P < 0.01) and Tyrosol (50 μg.mL-1, P < 0.05) could obviously improve the viability of AHH-1after radiation.

Conclusions: Results showed that glycosides or alcohols from Rhodiola had anti-radiation activities on AHH-1, and at concentration of 25 μg.mL-1 Rosavin had the most excellent anti-radiation effect in six components.