Interaction proteomics analysis of REC-1 by mass spectrometry identifies partners belonging to nucleosome core complex

M. I. Petalcorin. PAPRSB Institute of Health Sciences, Universiti Brunei Darussalam, Gadong, Brunei Darussalam.

Double-strand breaks (DSBs) are deleterious DNA lesions to our genome exploited in cancer radiotherapy. Although harmful, DSBs are naturally induced during meiosis essential for exchange of genetic materials between homologous chromosomes. REC-1 is required for normal distribution of meiotic crossovers along the chromosome. The positioning of crossover may be associated with the generation of DSBs, in which, REC-1 plays an important role requiring additional accessory partners to promote efficient DNA double strand break formation. We identified by interaction proteomics using mass spectrometry of phosphorylated purified REC-1 a multitude of proteins that can potentially assemble into multimeric complexes. These findings have spotlighted a new set of proteins involved in nucleosome remodeling suggesting interactive pathways connecting nucleosome remodelling, DSB formation and meiotic crossover positioning. Further investigation into these interacting pathways may give insight as to how meiotic patterns are generated with great potential of identifying targets for anti-cancer therapy.